Article Correlating Structure and Function of Battery Interphases at Atomic Resolution Using Cryoelectron Microscopy Yuzhang Li,1,4,5,* William Huang,1,4 Yanbin Li,1,4 Allen Pei,1 David Thomas Boyle,2 and Yi Cui1,3,* SUMMARY Battery decay and failure depend strongly on the solid electrolyte interphase Bactofilins are a recently discovered class of cytoskeletal proteins of which no atomic-resolution structure has been reported thus far. Evaluation of Higher Order Structure of Biologics at Atomic Resolution using NMR. Upon atomic resolution refinement, the solutions still showed some sterical clashes and were therefore not included in the top 10 submitted models (Figure 3C). The atomic resolution structure has been refined anisotropically using SHELXL96. Forschungsverbund Berlin. @article{osti_1351799, title = {Atomic-resolution structure of a disease-relevant Aβ(1-42) amyloid fibril}, author = {Wälti, Marielle Aulikki and Ravotti, Francesco and Arai, Hiromi and Glabe, Charles G. and Wall, Joseph S. and Böckmann, Anja and Güntert, Peter and Meier, Beat H. and Riek, Roland}, abstractNote = {Amyloid-β (Aβ) is present in humans as a 39- to 42-amino acid residue . CiteSeerX - Document Details (Isaac Councill, Lee Giles, Pradeep Teregowda): ABSTRACT A 1.10-Å atomic resolution X-ray structure of human fibroblast growth factor 1 (FGF-1), a member of the -trefoil superfold, has been determined. 598 Acta Cryst. Haoyu Jiang 1, Jizhen Qi 2, Dongchang Wu 5, Wei Lu 2,3, Jiahui Qian 3, Haifeng Qu 3, Yixiao Zhang 1, Pei Liu 4, Xi Liu 1 & Liwei Chen 1,2 Nano Research volume 14, pages 4802-4807 (2021)Cite this article To this end the crystal structure of reduced azurin II has been determined at an atomic resolution of 1.13 Å and is presented here. 2016 Aug 3;138(30):9663-74. doi: 10.1021/jacs.6b05129. 1g) reveal an isosceles lattice with in-plane constants measured as a 1 = 3.4 Å . In other words, this high-resolution method detects individual atoms and how they are arranged within a nanoparticle. Image Matching for Computer Vision in Atomic-Resolution Electron Microscopy, In progress (2020) Structure Prototypes, Supercells, and Image Simulation. between local atomic structure of active-site and the function of the material has emerged. Structure Analysis of GaN Thin Film with Inversion Domains by High Voltage Atomic Resolution Microscopy 1543 3. Atomic Resolution Structure of the Oncolytic Parvovirus LuIII by Electron Microscopy and 3D Image Reconstruction Viruses . An atomic-resolution structure of the CA hexamer was derived by integrating the NMR restraints and the low-resolution (8.0 Å) electron density map of a CA hexamer unit from tubular assemblies. took a unique advantage of the optical system by combining the Volta phase plate and Cs-corrector in a modern TEM to collect high-resolution micrographs of frozen-hydrated apo-ferritin in over-focus imaging conditions and determine the structure of apo-ferritin at 3.0 Å resolution. Atomic Resolution Structure of Monomorphic Aβ42 Amyloid Fibrils J Am Chem Soc. At resolutions better than 4 Å, atomic model building starts becoming possible but the direct visualization of true atomic positions in protein structure determination requires significantly higher (< 1.5 Å) resolution, which so far could not be attained by cryo-EM. Figure 1. In 1986, Binnig and Quate demonstrated for the first time the ideas of AFM, which used an ultra-small probe tip at the end of a cantilever (Phys. In solid-state NMR, there is no limit on the mass of the molecule, but issues with signal overlap and broad signals become worse with increasing mass. The need of high-resolution techniques is overall regarded as urgent for Higher Order Structure (HOS: secondary, tertiary and quaternary structure) September 27, 2018. Atomic-resolution structure of a disease-relevant Aβ(1-42) amyloid fibril Marielle Aulikki Wälti , a, 1 Francesco Ravotti , a, 1 Hiromi Arai , b Charles G. Glabe , b, c Joseph S. Wall , d Anja Böckmann , e, 2 Peter Güntert , a, f, g Beat H. Meier , a, 2 and Roland Riek a, 2 14 This was followed by the 3.2 Å resolution structure of catalase, which was determined from a single nanocrystal only 8 protein layers thick, 17, 51 and Ca 2+ /ATPase. For more than two decades, researchers have been trying to develop field emission . Sci Adv. 2017 Oct 30;9(11):321. doi: 10.3390/v9110321. The Nanostructure Fabrication and Measurement Group is developing critical-dimension and traceable microscope systems to calibrate probe tips and microscopy standards, and measure diverse devices ranging from waveguides to nanoparticles. The researchers call their development 3D SINGLE (Structure Identification . Fan et al. Atomic resolution structures (beyond 1.20 Å) at ambient temperature, which is usually hampered by the radiation damage in synchrotron X-ray crystallography (SRX), will add to our understanding of the structure-function relationships of enzymes. 600 random (unique) structure prototypes from entries in the ICSD were rotated 4 times such that the resulting viewing angle coincided with a unique . The key to success was combining two methods - solid-state NMR and cryo-electron microscopy. For many years, electron cryo-microscopy (cryo-EM) has held the promise that macromolecular structure determination should in principle be possible to near-atomic resolution from only several thousand projection images of isolated particles in ice (Henderson, 1995; Glaeser, 1999).However, cryo-EM single-particle reconstructions in which protein main-chains may be traced reliably or individual . The final model of HBP consists of 221 amino-acid residues of 225 possible, three glycosylation units, one chloride ion, 15 precipitant ethanol molecules and 323 water molecules. Atomic resolution structure of human α-tubulin acetyltransferase bound to acetyl-CoA Michael Taschner, Melanie Vetter, and Esben Lorentzen1 Department of Structural Cell Biology, Max Planck Institute of Biochemistry, D-82152 Martinsried, Germany Authors Michael T Colvin 1 . Determining the 3-D structure of phages at atomic resolution More information: Maximilian Zinke et al. (1998). Marielle Aulikki Wälti, Francesco Ravotti, Hiromi Arai, Charles G. Glabe, Joseph S. Wall, Anja Böckmann, Peter Güntert, Beat H. Meier, and Roland Riek. 56, p 930). 1,2, Lorena Geilen. Structure Retrieval at Atomic Resolution in the Presence of Multiple Scattering of the Electron Probe Hamish Brown, Zhen Chen, Matthew Weyland , C. Ophus, Jim Ciston, L. J. Allen, Scott Findlay School of Physics and Astronomy An atomic-resolution STM image of the ML film (Fig. Serial femtosecond crystallography (SFX) has attracted surging interest by providing a route to bypass such challenges. It is several times larger than the largest poly-merase, and 100 times larger than lysozyme, the first enzyme to be understood at atomic resolution. Keywords Development of a high-energy-resolution, LaB6 nanowire-based field emission gun: Electron source enables atomic resolution TEM observation. Phages have become a . Researchers at the University of Tokyo have combined novel techniques to record the first-ever atomic resolution video of salt crystals as they form in real-time. CiteSeerX - Document Details (Isaac Councill, Lee Giles, Pradeep Teregowda): An X-ray structure of l-asparaginase from Erwinia chrysanthemi (ErA) has been re®ned at 1 A Ê resolution to an R factor of below 0.1, using data collected on a synchrotron source. Atomic Resolution Cryo-EM Structure of B-galactosidase. Epub 2016 Jul 14. Application of this dataset to image retrieval tasks can be found in: Schwenker, E. et al. Data were collected in two passes, differing in the placement of the detector and the speed of data collection. At resolutions better than 4 Å, atomic model building starts to become possible, but the direct visualization of true atomic positions in protein structure determination requires much higher (better than 1.5 Å) resolution, which so far has not been attained by cryo-EM. Utilizing atomic-resolution electron microscopy, we reveal the presence and structure of 180°-type charged head-to-head and tail-to-tail domain walls passing throughout the thin film. In order to compare to the experimen- Atomic-resolution structure imaging for CS materials is usually done using a [110] projection since two sets of {111}-type lattice planes, which are the planes most commonly involved in structural defects such as stacking faults, are then projected edge-on to the incident beam direction. The high-resolution structure of the thermophilic LH1-RC provides the first atomic view of the organization and assembly of this large membrane protein-pigment super-complex, which revealed the detailed pigment arrangement, an intact RC structure, and some unique features such as the exchange routes for the bound Q B as well as for the . During the determination of the crystal structural of the full-length membrane protein ecYajR which had a medium resolution (3.15 Å), we determined the atomic-resolution crystal structure of the isolated YAM domain at 1.07 Å as well as its nuclear magnetic resonance (NMR) structure.We demonstrate that the YAM domain forms a homodimer in the crystal and possibly in solution as well. Atomic Resolution Structure of the Oncolytic Parvovirus LuIII by Electron Microscopy and 3D Image Reconstruction . We report methods to account for radiation damage and local changes in defocus and image drift, enabling visualization of atomic resolution features in a cryo-EM density map of inhibitor-bound -galactosidase, and derivation of atom-specific measures of local flexibility of the bound inhibitor using constrained molecular dynamics simulations. To explore the potential of the new technologies to yield even higher-resolution reconstructions, we tested a mouse apoferritin sample. Qualitative measures. a Laboratorium für Physikalische Chemie, Eidgenössische Technische Hochschule Zürich, 8093 Zurich . 1,2, Sujata Halder. Retrieved January 13, 2022 from www . The direct visualization of atom positions is essential for understanding . Bartesaghi et al. Print 2021 Jan. Atomic-resolution structure determination is therefore essential to understand chemical reaction mechanisms because it is the slight deviation from normal chemistry that drives chemical reactions . Deposition of atomic or near-atomic-resolution structures in the Protein Data Bank according to different experimental methods. ( A) The number of structures deposited annually is shown between 2010 and the end of 2019, based on X-ray diffraction, cryo-electron microscopy (EM), and NMR methods. Shen Yu 1,2, Allegra Vit 3, Sean Devenish 4,5, H Khris Mahanty 4, Aymelt Itzen 1, Roger S Goody 1 & Wulf Blankenfeldt 1,3 Scientists at Scripps Research, Florida have determined the near-atomic-scale structure of an unusual brain-cell receptor called GPR158, which has been linked to depression and anxiety. Data-collection statistics for the atomic resolution structure of ErA. Atomic-resolution holographies 2.1. Credit: Barth van Rossum, FMP. The NIMS-Jeol team claim this is the highest resolution ever recorded for non-monochromatic electron guns, with a high current stability of 0.4%. The atomic resolution structure has been refined with individual anisotropic atomic thermal parameters. Co-ordinates and structure factors have been deposited in the RCSB Protein Data Bank with accession codes 2ccw and r2ccwsf respectively. Atomic-resolution characterization on the structure of strontium doped barium titanate nanoparticles. 2021 Jan 29;7 (5):eabe6679. Figure 1 is a typical high-resolution image of GaN taken by HVARM. The 3D atomic positions of individual nanoparticles can be extracted with a precision of 0.02 nm — six times smaller than the smallest atom: hydrogen. In collaboration with colleagues from Forschungszentrum Jülich and Jena University Hospital, they have now revealed the 3D structure of this crucial phage component in atomic resolution. Letters, 1986, Vol. The atomic resolution structure of crambin is an example of the crystallization and structure determination of a protein with micro-heterogeneity in its primary sequence (Hendrickson & Teeter, 1981; Teeter & Roe, 1993). Hence new science to investigate the physics of the property induced by a non-periodic local atomic structure was born. Request PDF | Atomic-resolution protein structure determination by cryo-EM | Single-particle electron cryo-microscopy (cryo-EM) is a powerful method for solving the three-dimensional structures of . 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